Brandt Instruments is the recipient of the MesaLabs 2016 Summit Club Award

Bios International is Now A Division of MesaLabs

Brandt Instruments-US Master Distributors for MesaLabs | Bios Definer 220 DryCal primary air flow calibrators with automatic temperature & pressure compensation | dry piston calibrator technology
Brandt Instruments Representing MesaLabs
Bios Definer 220 Primary Flow Calibrator
Using DryCal® Technology

Bios International is now a division of MesaLabs
MesaLabs Bios DryCal

Definer 220 Primary Flow Calibrator
Automatic Temperature & Pressure Compensation
Air Flow Calibrators with DryCal® Technology

End of Life Notice: Effective January 16th, 2017, MesaLabs discontinued the Definer 220. The Definer 220 has been directly replaced by the new Defender 530+. MesaLabs will continue to accept RMA requests for service and calibration of the Definer 220.

                                                        Purchase Order Form MesaLabs Definer 220 End-of-Life Notice

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MesaLabs DryCal Definer 220 Primary Flow Calibrator

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MesaLabs Bios Definer 220 Precision Calibrator Presentation

Definer 220 - Click link to view instrument close-up


 The MesaLabs Bios DryCal Definer 220 series calibrator has been discontinued by MesaLabs and has been directly replaced by the Defender 530+ effective January 16, 2017.

The 530+ calibrators have the same flow rates as the Definer 220 from 5 cc to 30 liters, are true primary flow standards which allows users to verify gas flow rates, in the field or in the lab, to an accuracy of 1% percent for readings standardized for temperature and pressure and 0.75% in volumetric flow.

Unlike thermal mass flow meters, the Definer 220 and Defender 530+ positive displacement technology provides immediate indication of actual volumetric gas flow rate accurately and independently of gas type.  Integrated temperature and pressure sensors in the flow stream allow users to automatically standardize volumetric flow readings to standard conditions, for traceable verifications of mass flow devices.

A versatile, push-button flow meter, the Defender 530+ is a primary flow standard which enables the user to accurately calibrate a variety of instruments while maintaining an ISO 17025-traceable audit trail.

MesaLabs' Bios DryCal Definer 220 - Overview


  • Standardized: ±1.0%, Volumetric ±0.75%
  • Proven percent of reading accuracy is supported by ISO 17025 accreditation by NVLAP of NIST
  • Integrated temperature sensors and pressure transducers placed directly in the flow stream for optimum standardized results recording
  •  Choose between volumetric or standardized flow units – (mL/min, L/min, cc/min, cf/min)
  •  Include up to one hundred readings in a group average
  •  Take individual readings, continuous hands-free readings, or set to read in time intervals anywhere
     from one minute to sixty minutes between readings


  • The DryCal® Pro software is available for the Definer with an integrated serial PC interface for easy transfer of flow data to a text file, with automatic recording and graphing of flow measurements. These records can also be easily opened in a variety of off-the-shelf database applications, including Microsoft Excel or Acces

Proven DryCal Technology Defender 510/520 Specifications

Definer 220 Specifications

Size 5.5"H x 6"W x 3"D
140 mm x 150 mm x 75 mm
Weight 29 oz. / 820 g
Flow Ranges  
Definer 220 (L) Low Flow 5 to 500 scc/min
Definer 220 (M) MediumFlow 50 - 5,000 scc/min 
Definer 220 (H) HighFlow 300 - 30,000 sccl/min
Accuracy At gas pressure of 760 mmHg, gas temperature of 25°C with standard temperature set to 0°C.


User-Selectable Measurement Units
Volumetric Flow mL/min, L/min, cc/min, cf/min
Standard Flow smL/min, sL/min, scc/min, scf/min
Pressure mmHg, PSI, kPa
Temperature ºC, ºF
Approximate Time per Reading 1-15 seconds
Gas Compatibility Use with non-corrosive, non-condensing, non-combustible gases, less than 70% humidity
Flow Modes Suction or Pressure
Measurement Cell Style Integrated
Temperature & Pressure Sensors In the flow stream
Press: 3.5 mmHg (typical), 7.0 mm (max)
Temp: 0.8 °C (typical) 1.3 °C (max)
Reading Styles Single (manual), Continuous or Burst, with averaging function user-selectable from 1 to 100 measurements
AC Adapter/Charger 12 DC, >250 ma, 2.5 mm, center positive
Battery System 6V rechargeable, sealed lead-acid, 6-8 hours typical operation
Battery Operational Time  3 hours backlight on, 8 hours backlight off
Pressure & Suction Fittings ¼” ID Swagelok® compression fittings,
3/8" on High Model
Warranty Instrument: 1 year, Battery: 6 months
Storage Temperature 0-70 °C
Ambient Humidity 0-70% - non-condensing
Operating Pressure  (Absolute) 15 PSI
Display Backlit graphical LCD
Data Port
Serial RS-232 (for use with Optimizer software)
Data Cable 1 meter (Defender Data Port to PC Serial Port)
Protective Case Soft side or Pelican case available
Laboratory Accreditation MesaLabs: ISO 17025 accreditation
Instrument Certification RoHS and CE Compliant

Adobe Acrobat pdf format - Bios Gas  Flow Bench Bios_Definer 220 Met Lab Calibrator_Brochure

Kit Options

  Proven DryCal Technology

       Bios Definer 220 Series Calibrator Kit Options & Pricing

The New Bios Defender 530+, which takes the place of the Definer 220, Includes: Instrument, 100-240V AC Power Adapter/Charger, Serial Cable, Plastic Leak Test Cap (1), NIST-Traceable Calibration Certificate, Mini-Manual, and Swagelok® fittings (models L&M, ¼"; model H only, 3/8") and instructions for downloading the free DryCal® Pro Software. Included is a barb fitting adapter for use with soft tubing.

* Calibrators come standard with US chargers
Adapter plugs available for use in Australia, UK or Europe
For each additional plug add $10.00

NOTE: The Definer 220 has been replaced by the Defender 530+ Calibrators

Part Number


Flow Range

200-530+L Defender 530+ Low Flow 5 to 500 smL/min
200-530+M Defender 530+ Medium Flow 50 to 5000 sml/min
200-530+H Defender 530+ High Flow 300 to 30,000 sml/min


Brandt instruments, Inc. are the distributor for MesaLabs only in the US

Overseas customers please contact your local distributor for pricing which includes international shipping charges, import duties and local taxes.

Purchase Order Form     Download pdf Purchase Order Form
   Purchase Order Form    Download Word Purchase Order Form

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MesaLabs Bios Definer 220 used on Met-One Station

Definer 220 used on
Met One Station

Terms for Instrument and Accessory Returns:
NOTE: Any returns will incur a 30% restocking fee on the list price of the item. To return any instruments or accessories you must request a Return Authorization number prior to returning anything to the factory for credit. This Return Authorization number must be prominently displayed on the outside of the shipping box. All products returned to MesaLabs must be sent freight pre-paid, in new condition and in the original cartons with all unopened software, accessories and manuals. The sender is responsible for any carrier loss or damage.

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    18568 Oak Grove Parkway 
    Prairieville, LA  70769 
Phone: 225-673-6776
    Fax: 225-673-6725

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